Detection of Apoptotic Cells Using Immunohistochemistry
نویسندگان
چکیده
منابع مشابه
Detection of apoptotic cells using immunohistochemistry.
Immunohistochemistry is commonly used to show the presence of apoptotic cells in situ. In this protocol, B-cell lymphoma cells are injected into recipient mice and, on tumor formation, the mice are treated with the apoptosis inducer vorinostat (a histone deacetylase inhibitor). Tumor samples are fixed and sectioned, and fragmented DNA (a feature of apoptotic cells) is end-labeled by terminal de...
متن کاملDenovirus Antigen Detection in Paraffinized Lung Sections of Pneumonic Goat Lungs Using Immunohistochemistry
BACKGROUNDS: Diseases affecting the respiratory tract of sheep and goats are one of the most important factors which limit production of these species on a world-wide basis. OBJECTIVES: The main goal of this study was to determine Adenovirus (AdV) antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic goats, using immunohistochemistry (IHC) staining method. METHODS: For this purpo...
متن کاملBHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry
Background: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. METHODS: For this purpose, the lungs of 4079 sheep, which...
متن کاملdetection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts
in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...
Detection of apoptotic fetal cells in plasma of pregnant women.
other laboratories (6), it makes economic sense to consolidate to one platform that can perform multiple assays without operator intervention. We have extended this method to include measurements of acetyl carnitine (data not shown). In this case, a mixture of labeled carnitines are added to plasma, and concentrations are calculated from calibration curves for free, acetyl, and total carnitine....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2014
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot082537